github.com/iwc-workflows/pseudobulk-worflow-decoupler-edger/main

Performs differential gene expression analysis on single-cell data using a pseudobulk approach. Aggregates cell-level counts from an annotated AnnData object by cell type or other metadata using Decoupler, then applies edgeR for robust statistical testing between conditions. Includes data preprocessing steps for compatibility with edgeR and generates interactive volcano plots to visualize significantly differentially expressed genes.